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CRISPR knockout screens outperform shRNA and CRISPR-interference screens in a side-by-side comparison. 
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BigField GEG Tech's insight:
Challenges in the delivery of small interfering RNA (siRNA) have hampered clinical translation. Polymeric or periodic short hairpin RNAs (p-shRNAs) are a recent development that can potentially address these delivery barriers by showing improved stability and complexation to enable nanoparticle packaging. Here The scientists modify these biomacromolecules packaging via structural and sequence engineering coupled with selective enzymatic digestion to generate an open-ended p-shRNA (op-shRNA) that is cleaved over ten times more efficiently to yield siRNA. The op-shRNA induces considerably greater gene silencing than p-shRNA in multiple cancer cell lines up to nine days. Op-shRNA provides an RNAi platform that can potentially be packaged and delivered efficiently to disease sites with higher therapeutic efficacy.
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BigField GEG Tech's insight:
The authors have developed a nanocapsule delivery technology that encapsulates small DNA molecules encoding RNAs into a small polymer nanocapsule. They successfully tested this delivery system with shRNA and CRISPR to target CCR5.
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BigField GEG Tech's insight:
In this review the author focus on the novel Cas9-based gene editing technologies, which look set to replace the established shRNA approach to target identification and are proving to be a powerful addition to the gene editing toolbox for model generation.
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This video describes how vectorizing shRNA and deliver it through lentiviral vectors can improve a research project.
BigField GEG Tech's insight:
This video describes how vectorizing shRNA and deliver it through lentiviral vectors can improve your research project. http://geg-tech.com/rna-interference.html
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BigField GEG Tech's insight:
Resources for genome-scale loss-of-function screens in mammalian cells have progressed rapidly, particularly with RNA interference (RNAi)-based libraries and, more recently, with CRISPR–Cas9-based libraries. A new parallel screening study leverages the complementary strengths of each system to dissect antiviral drug mechanisms and to identify potential combination therapy strategies.
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BigField GEG Tech's insight:
A new way to avoid genotoxicity? Several types of CRISPR–Cas adaptive immune system have been described, each with a unique mechanism and protein composition. DNA cleavage by type III-A CRISPR–Cas systems relies on a complex known as Cas10–Csm and requires transcription of the target DNA. Type III-A CRISPR–Cas systems are also capable of degrading RNA, although why they should have an RNase function is not known. Jiang et al. now show that some phage targets can delay the transcription-dependent DNA cleavage mechanism of type III-A CRISPR–Cas systems but that CRISPR RNases prevent the replication of these phages.
Martina Hrabinová's curator insight,
March 31, 2016 5:14 AM
A new way to avoid genotoxicity? Several types of CRISPR–Cas adaptive immune system have been described, each with a unique mechanism and protein composition. DNA cleavage by type III-A CRISPR–Cas systems relies on a complex known as Cas10–Csm and requires transcription of the target DNA. Type III-A CRISPR–Cas systems are also capable of degrading RNA, although why they should have an RNase function is not known. Jiang et al. now show that some phage targets can delay the transcription-dependent DNA cleavage mechanism of type III-A CRISPR–Cas systems but that CRISPR RNases prevent the replication of these phages.
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BigField GEG Tech's insight:
CRISPR/Cas can be exploited not only to knockout genes but also to up- or down-regulate gene transcription—in some cases in a multiplex fashion. This provides a powerful tool for studying the interaction among multiple signaling cascades in the same genetic background.
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BigField GEG Tech's insight:
Scientists describe the use of lentiviral shRNA to identify the involvement of an enzyme that syntheses d-serine to modulate glutamate- N-methyl-d-aspartate receptor- in regulating rat cerebellar granule neurons apoptosis. geg-tech.com
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Differential knockdown of TGF-beta ligands in a three-dimensional co-culture ...
BigField GEG Tech's insight:
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High-throughput genetic screens have become essential tools for studying a wide variety of biological processes. Here the scientists experimentally compare systems based on clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) or its transcriptionally repressive variant, CRISPR-interference (CRISPRi), with a traditional short hairpin RNA (shRNA)-based system for performing lethality screens. They find that the CRISPR technology performed best, with low noise, minimal off-target effects and consistent activity across reagents.