Genetic Engineering Publications - GEG Tech top picks

A key goal of developmental biology is to understand how a single cell is transformed into a full-grown organism comprising many different cell types. Here th scientists present LINNAEUS (lineage tracing by nuclease-activated editing of ubiquitous sequences)—a strategy for simultaneous lineage tracing and transcriptome profiling in thousands of single cells. 

The method describes CRISPR droplet sequencing (CROP-seq) enables pooled CRISPR screens with single-cell transcriptome resolution, which will facilitate high-throughput functional dissection of complex regulatory mechanisms and heterogeneous cell populations.