The authors enhanced the integration site-specificity of the phiC31 integrase-based vector using a sequence-specific DNA-binding protein containing Gal4 and LexA DNA-binding motifs. In human cells, they showed thanks to quantitative PCR that integration around the LexA operator was 26-fold higher than with the control.
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The authors enhanced the integration site-specificity of the phiC31 integrase-based vector using a sequence-specific DNA-binding protein containing Gal4 and LexA DNA-binding motifs. In human cells, they showed thanks to quantitative PCR that integration around the LexA operator was 26-fold higher than with the control.
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