Research and publish the best content.
Get Started for FREE
Sign up with Facebook Sign up with X
I don't have a Facebook or a X account
Already have an account: Login
Genetic Engineering Publications - GEG Tech top picks
36.6K views |
+2 today
 Your new post is loading...
Your new post is loading...
Scoop.it!
Efficient generation of plants carrying mutations in multiple genes remains a challenge.
BigField GEG Tech's insight:
Here, the scientists describe MISSA 2.0 (multiple-round in vivo site-specific assembly 2.0), an extensively updated toolkit for assembly of two or more CRISPR/Cas systems. They developed a novel suicide donor vector system based on plasmid RK2, which has much higher cloning capacity than the original, plasmid R6K-based system. 
Scoop.it!
BigField GEG Tech's insight:
Scientists designed a new approach which provides a synthetic biology platform for targeting and controlling bladder cancer cells in vitro.
|
Scoop.it!
BigField GEG Tech's insight:
Here, scientists describe the de novo synthesis of synthetic yeast chromosome V (synV) in the “Build-A-Genome China” course, perfectly matching the designer sequence and bearing loxPsym sites, distinguishable watermarks, and all the other features of the synthetic genome. They generated a ring synV derivative with user-specified cyclization coordinates and characterized its performance in mitosis and meiosis.
Scoop.it!
BigField GEG Tech's insight:
The authors review on the present regulation of CRISPR and discuss the translational aspect of genome engineering research and patient autonomy with respect to the “right to try” potential novel non-germline gene therapies.
|
The leakiness of commonly used genetic components can make the construction of complex synthetic circuits difficult.