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Genetic Engineering Publications - GEG Tech top picks
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BigField GEG Tech's insight:
Here, the auhtors established a new method for generating pig cell lines using the Sleeping Beauty (SB) transposon-mediated ectopic expression of porcine telomerase reverse transcriptase (pTERT). After passage for more than 40 generations, the cell line retained stable expression of ectopic pTERT and continuous growth potential. These data suggest that the new method established is useful for generating pig cell lines without viral sequence and antibiotic resistant gene. 
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To overcome the low CRISPR-Cas9 editing efficiency at the TERT locus, the scientists develop a two-step “pop-in/pop-out” strategy to enrich cells that underwent homologous recombination (HR). This method provides a useful tool for studying telomerase biology, and suggests a general approach to edit loci with low targeting efficiency and to purify and visualize low abundance proteins.
www.geg-tech.com/Vectors