Genetic Engineering Publications - GEG Tech top picks

In this work, the scientists describe FusX, a streamlined Golden Gate TALE assembly system that (1) is backward compatible with popular TALE backbones, (2) is functionalized as a single-tube 3-day TALE assembly process (3) requires only commonly used basic molecular biology reagents (4) and is cost-effective. Over 100 TALEN pairs have been successfully assembled using FusX, and 27 pairs were quantitatively tested in zebrafish with each showing high somatic and germline activity.