Genetic Engineering Publications - GEG Tech top picks

Here, the authors construct logic AND circuits by integrating multiple split dCas9 domains, which is useful to reduce the size of synthetic circuits. In addition, we engineer sensory switches by exchanging split dCas9 domains, allowing differential regulations on one gene, or activating two different genes in response to cell-type specific microRNAs. Therefore, they provide a valuable split-dCas9 toolkit to engineer complex transcription controls, which may inspire new biomedical applications.