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Genetic Engineering Publications - GEG Tech top picks
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The authors established a CRISPR-Cas9 method enables rapid and efficient genome manipulation and unlocks the CRISPR toolset for use in fission yeast. 
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Here, scientists describe the de novo synthesis of synthetic yeast chromosome V (synV) in the “Build-A-Genome China” course, perfectly matching the designer sequence and bearing loxPsym sites, distinguishable watermarks, and all the other features of the synthetic genome. They generated a ring synV derivative with user-specified cyclization coordinates and characterized its performance in mitosis and meiosis.