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Scoop.it!
UT Southwestern researchers today report the first use of CRISPR genome-wide screening to identify a gene that helps cells resist West Nile virus, dengue fever, Zika virus, and yellow fever. 
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Scoop.it!
BigField GEG Tech's insight:
In this work the authors report a pipeline for the rapid design, assembly, and validation of cell-free, paper-based sensors for the detection of the Zika virus RNA genome. By linking isothermal RNA amplification to toehold switch RNA sensors, they detect clinically relevant concentrations of Zika virus sequences and demonstrate specificity against closely related Dengue virus sequences. When coupled with a novel CRISPR/Cas9-based module, the sensors can discriminate between viral strains with single-base resolution.
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In a study published in Nature Microbiology, the team led by Dr. John Schoggins, Assistant Professor of Microbiology, used the cutting-edge CRISPR technology to identify the IFI6 gene as a potent antiviral gene targeting flaviviruses. The researchers then used traditional cell culture studies to confirm the gene’s role in protecting against infection by Zika, West Nile, dengue, and yellow fever viruses.