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Genetic Engineering Publications - GEG Tech top picks
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BigField GEG Tech's insight:
The authors used zinc finger nucleases (ZFNs) directed against the gene encoding human PD-1 (PDCD-1) to gene-edit melanoma Tumor Infiltrating Lymphocyte (TIL). They show that their clinical scale TIL production process yielded efficient modification of the PD-1 gene locus, with an average modification frequency of 74.8% of the alleles in a bulk TIL population, which resulted in a 76% reduction in PD-1 surface-expression. 
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BigField GEG Tech's insight:
In this study, the authors design high efficiency TALEN and ZFN to target two safe harbor sites on chromosome 13 and 19 to generate reporter systems while retaining pluripotent characteristics. They demonstrate the possibility to use a Cre-recombinase induced cassette exchange strategy to rapidly exchange reporter cassettes to develop new reporter lines in the same isogenic background at high efficiency. The results provide a novel platform for rapidly developing custom single or dual reporter systems for screening assays.
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BigField GEG Tech's insight:
This review focuses on the structure, design, and applications of ZF DNA binding domains (ZFDBDs). ZFDBDs are relatively small and have been shown to penetrate the cell membrane without additional tags suggesting that they could be delivered to cells without a DNA or RNA intermediate. Advanced algorithms that are based on extensive knowledge of the mode of ZF/DNA interactions are used to design the amino acid composition of ZFDBDs so that they bind to unique sites in the genome.
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BigField GEG Tech's insight:
Xenotransplantation has been proposed as a solution to the shortage of suitable human donors for transplantation and pigs are currently favoured as donor animals. However, xenotransplantation may be associated with the transmission of zoonotic microorganisms.The zinc finger nuclease (ZFN) technology was used to reduce the risk of horizontal PERV transmission and to knock out as many as possible proviruses.
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BigField GEG Tech's insight:
Friedreich’s ataxia (FRDA) is an autosomal recessive neurological disease caused by expansions of GAA repeats in intron 1 of the frataxin (FXN) gene. The authors generated zinc finger nuclease (ZFN) to excise the expanded GAA repeats. This correction persisted during reprogramming of ZFN-edited fibroblasts to induced pluripotent stem cells (iPSCs) and subsequent differentiation into neurons. They observed that he expression of FRDA biomarkers were reversed in ZFN-corrected neuronal cells.
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“Which technology should I use in my experiment?” This review offers a practical resource to compare and contrast these technologies, guiding the investigator when and where to use this fantastic array of powerful tools.
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