The CRISPR-Cas9 system has recently emerged as a versatile tool for biological and medical research.
Get Started for FREE
Sign up with Facebook Sign up with X
I don't have a Facebook or a X account
Your new post is loading...
Your new post is loading...
|
Here, the authors evaluated the cleavage activities of 218 sgRNAs and found that nucleotides at both PAM-distal and PAM-proximal regions of the sgRNA are significantly correlated with on-target efficiency. They also demonstrated that the genomic context of the targeted DNA, the GC percentage, and the secondary structure of sgRNA are critical factors contributing to cleavage efficiency. This study reveals important parameters for the design of sgRNAs with high on-target efficiencies, especially in the context of high throughput applications.