This article describes the protocol for the delivery of reagents for targeted genome editing to CD34(+) hematopoietic stem/progenitor cells (HSPCs). While optimization steps might be needed for each specific application with respect tonuclease and donor template amount, adherence to this protocol will serve as an excellent starting point for this further work.
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This article describes the protocol for the delivery of reagents for targeted genome editing to CD34(+) hematopoietic stem/progenitor cells (HSPCs). While optimization steps might be needed for each specific application with respect tonuclease and donor template amount, adherence to this protocol will serve as an excellent starting point for this further work.