CIRCLE-seq is an in vitro assay for selectively sequencing off-target sites cleaved by Cas9-sgRNA in genomic DNA. It sensitively profiles genome-wide off-target cut sites and characterizes the contribution of SNPs to these cleavage events.
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CIRCLE-seq is an in vitro assay for selectively sequencing off-target sites cleaved by Cas9-sgRNA in genomic DNA. It sensitively profiles genome-wide off-target cut sites and characterizes the contribution of SNPs to these cleavage events.