Here the authors report that the CRISPR/Cas9 technology can be used to efficiently generate stable targeted gene mutations in microalgae, using the marine diatom Phaeodactylum tricornutum as a model species. Their vector design opens for rapid and easy adaption of the construct to the target chosen. To screen for CRISPR/Cas9 mutants they employed high resolution melting based PCR assays, mutants were confirmed by sequencing and further validated by functional analyses.
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Here the authors report that the CRISPR/Cas9 technology can be used to efficiently generate stable targeted gene mutations in microalgae, using the marine diatom Phaeodactylum tricornutum as a model species. Their vector design opens for rapid and easy adaption of the construct to the target chosen. To screen for CRISPR/Cas9 mutants they employed high resolution melting based PCR assays, mutants were confirmed by sequencing and further validated by functional analyses.