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Genetic Engineering Publications - GEG Tech top picks
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BigField GEG Tech's insight:
Genome editing using designer nucleases such as TALENs or the CRISPR-Cas9 system is hampered by a lack of methods to detect and quantify the products. Here the authors present GEF-dPCR, a droplet-based digital PCR method for assessing gene-editing frequencies. 
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In this work the authors report a pipeline for the rapid design, assembly, and validation of cell-free, paper-based sensors for the detection of the Zika virus RNA genome. By linking isothermal RNA amplification to toehold switch RNA sensors, they detect clinically relevant concentrations of Zika virus sequences and demonstrate specificity against closely related Dengue virus sequences. When coupled with a novel CRISPR/Cas9-based module, the sensors can discriminate between viral strains with single-base resolution.