Genetic Engineering Publications - GEG Tech top picks

To extend the catalog of human core and context-dependent fitness genes, the authors of the study have developed a high-complexity second-generation genome-scale CRISPR-Cas9 gRNA library and applied it to fitness screens in five human cell lines. Theypresent a list of 1,580 human core fitness genes and describe their general properties. The rigorous identification of human cell line fitness genes using a high-complexity CRISPR-Cas9 library affords a high-resolution view of the genetic vulnerabilities of a cell.

The authors utilize CRISPR/Cas9 technology for targeted gene modification of four of the most commonly mutated colorectal cancer genes (APC, P53, KRAS and SMAD4) in cultured human intestinal stem cells. Upon xenotransplantation into mice, quadruple mutants grow as tumours with features of invasive carcinoma. Finally, combined loss of APC and P53 is sufficient for the appearance of extensive aneuploidy, a hallmark of tumour progression.

www.geg-tech.com/Vectors