Here, the scientists developed a ligation-independent cloning (LIC) assembly method for efficient and bias-free generation of large sgRNA libraries. They tested their selection constructs in HEK 293T cells and showed a median genome editing activity of 54.6% and their shRNA of this library to generate knockout cells was successful for 19 out of 19 genes tested.
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Here, the scientists developed a ligation-independent cloning (LIC) assembly method for efficient and bias-free generation of large sgRNA libraries. They tested their selection constructs in HEK 293T cells and showed a median genome editing activity of 54.6% and their shRNA of this library to generate knockout cells was successful for 19 out of 19 genes tested.
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