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The repair of a point mutation can be facilitated by combined activity of a single-stranded oligonucleotide and a CRISPR/Cas9 system.
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The repair of a point mutation can be facilitated by combined activity of a single-stranded oligonucleotide and a CRISPR/Cas9 system. In this study, the scientists provide a detailed analysis of allelic variance at and surrounding the target site. In one particular case, they report sequence alteration directed by a distinct member of the same gene family. Their data suggests that single-stranded DNA molecules may influence DNA junction heterogeneity created by CRISPR/Cas9.