A tool for genome-wide design of CRISPR guide RNAs reduces off-target effects and facilitates targeting of the non-coding genome.
Get Started for FREE
Sign up with Facebook Sign up with X
I don't have a Facebook or a X account
Your new post is loading...
Your new post is loading...
|
Here, scientists present GuideScan software for the design of CRISPR guide RNA libraries that can be used to edit coding and noncoding genomic regions. GuideScan produces high-density sets of guide RNAs (gRNAs) for single- and paired-gRNA genome-wide screens. They also show that the trie data structure of GuideScan enables the design of gRNAs that are more specific than those designed by existing tools.