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Genetic Engineering Publications - GEG Tech top picks
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BigField GEG Tech's insight:
In this study, the authors successfully produced double point mutations in rice acetolactate synthase gene (OsALS) and generated herbicide resistant rice lines by using TALENs and donor DNA carrying the desired mutations. The HR-mediated gene edits were heritable to the progeny of T1 generation. The edited T1 plants were as morphologically normal as the control plants while displayed strong herbicide resistance. The results demonstrate the feasibility of TALEN-mediated genome editing in rice and provide useful information for further genome editing by other nuclease-based genome editing platforms. 
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BigField GEG Tech's insight:
In this work, the scientists compre TALEN and CRISPR to target two loci within the EGFP gene. They find that the CRISPR system induced targeted genomic deletion more efficiently and precisely than TALENs. However, TALENs stimulated homology directed repair more efficiently than CRISPR system and caused fewer targeted genomic deletions. These data suggest that the choice of genome editing tool should be determined by the desired genome editing outcome. Such a rational approach is likely to benefit research outputs for groups working in fields as diverse as modification of cell lines, to animal models for disease studies, or gene therapy strategies.
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Genome editing of human ES/iPS cells has been limited by technical difficulties that result in a low efficiency of homologous recombination (HR) in human ES/iPS cells.
In this work, the authors demonstrated that RAD51 overexpression and valproic acid treatment enhanced biallelic-targeting efficiency in human ES/iPS cells regardless of the transcriptional activity of the targeted locus. Their findings would facilitate genome editing study using human ES/iPS cells.