Genetic Engineering Publications - GEG Tech top picks

The authors provide an online tool for the design of highly active sgRNAs for any gene of interest.

Indeed, they discovered sequence features that improved activity, including a further optimization of the protospacer-adjacent motif (PAM) of Streptococcus pyogenes Cas9. The results from 1,841 sgRNAs were used to construct a predictive model of sgRNA activity to improve sgRNA design for gene editing and genetic screens.

http://www.broadinstitute.org/rnai/public/analysis-tools/sgrna-design