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Genetic Engineering Publications - GEG Tech top picks
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BigField GEG Tech's insight:
In this study, the authors design high efficiency TALEN and ZFN to target two safe harbor sites on chromosome 13 and 19 to generate reporter systems while retaining pluripotent characteristics. They demonstrate the possibility to use a Cre-recombinase induced cassette exchange strategy to rapidly exchange reporter cassettes to develop new reporter lines in the same isogenic background at high efficiency. The results provide a novel platform for rapidly developing custom single or dual reporter systems for screening assays. 
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“Which technology should I use in my experiment?” This review offers a practical resource to compare and contrast these technologies, guiding the investigator when and where to use this fantastic array of powerful tools.
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