Here, the scientists present a new guide RNA prediction tool which can predict sgRNA activity across multiple CRISPR systems. In addition to predicting activity for Cas9 from S. pyogenes and S. thermophilus 1, they experimentally demonstrate that their algorithm can predict activity for Cas9 from S. aureus and S. thermophilus 3. They also have made available a new version of our software, sgRNA Scorer 2.0, which will allow users to identify sgRNA sites for any PAM sequence of interest.
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Here, the scientists present a new guide RNA prediction tool which can predict sgRNA activity across multiple CRISPR systems. In addition to predicting activity for Cas9 from S. pyogenes and S. thermophilus 1, they experimentally demonstrate that their algorithm can predict activity for Cas9 from S. aureus and S. thermophilus 3. They also have made available a new version of our software, sgRNA Scorer 2.0, which will allow users to identify sgRNA sites for any PAM sequence of interest.