Genetic Engineering Publications - GEG Tech top picks

Pioneering experiments to modify the genome of human pluripotent stem cells (hPS cells) utilizing site-specific double-strand break (DSB)-mediated genome engineering tools, including zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs), have paved the way to genome engineering in previously recalcitrant systems. However, these methods are technically cumbersome and require significant expertise, which has limited adoption. In this article, the authors describe commonly practiced methods for CRISPR endonuclease genomic editing of hPS cells into cell lines containing genomes altered by insertion/deletion (indel) mutagenesis or insertion of recombinant genomic DNA.